Aflatoxin B1 ELISA Test Kit

Product Description

REAGEN™ Aflatoxin B1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin B1 in cereals, meat/fish, feed, milk ,oils,peanuts and pistachios .The unique features of the kit are:
High recovery (>80%),rapid(10-40minutes), cost-effective extraction methods.
High sensitivity (0.05 ng/g or ppb).
A quick ELISA assay (less than 2 hours regardless of number of samples).
High reproducibility.

Procedure Overview

REAGEN™ Aflatoxin B1 ELISA Test Kit is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN™ Aflatoxin B1 ELISA Test Kit has the Aflatoxin B1acity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents Amount Storage
Aflatoxin B1 Antibody-Coated Plate 1x 96-well Plate (8 wells x 12 strips) 2-8C
Aflatoxin B1 Standards:
Negative control (white cap tube)
0.05ng/mL (yellow cap tube)
0.1ng/mL (orange cap tube)
0.2 ng/mL (pink cap tube)
0.4ng/mL (purple cap tube)
0.8 ng/mL (blue cap tube)
100 ng/mL (spiking, red cap tube)
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
0.8 mL
2-8C

2-8C

Aflatoxin B1 Antibody #1 8ml 2-8C *
HRP Conjugated Antibody #2 18mL 2-8C
20X Wash Solution 28 mL 2-8C
Stop Buffer 20 mL 2-8C
TMB Substrate 12 mL 2-8C
Concentrate of Feed Extraction Buffer (Optional) 60g 2-8C
Feed Clean Up Mix (Optional) 6g 2-8C

* If you are not planning to use the kit for over 3 months, store Aflatoxin B1 Antibody #1 and HRP-Conjugated Antibody #2 at -20C or in a freezer.

Sensitivity

Sample Type Detection Limit (ng/g or ppb)
Meat/fish/sereal/feed/seed/starch 1.0(Rapid Method); 2.5(Organic Solvent Method)
Meat/liver/kidney 0.5
Milk 0.5
Peanut butter 0.5
Vegetable/bean/peanut/olive oils 2.5

Specificity

Analytes Cross-Reactivity (%)
Aflatoxin B1 100.0
Aflatoxin B2 49
Aflatoxin M1 45
Aflatoxin M2 24
Aflatoxin G1 37
Aflatoxin G2 8